Title: Data of quantitative, ratiometric dual fluorescence reporter assay at the single-cell level

Type Dataset Kojima, Shin-ichiro, Borisy, Gary G. (2014): Data of quantitative, ratiometric dual fluorescence reporter assay at the single-cell level. Zenodo. Dataset. https://zenodo.org/record/8296

Authors: Kojima, Shin-ichiro (Department of Life Science, Faculty of Science, Gakushuin University, Tokyo 171-8588, Japan) ; Borisy, Gary G. (The Forsyth Institute, Cambridge, MA 02142, USA) ;

Links

• Item record in Zenodo
• Digital object URL

Summary

The data set shows the evaluation of a new dual fluorescence reporter assay using three test genes, vimentin, lamin A/C and Arp3. This fluorescence protein-based reported assay successfully predicts siRNA efficacy with high fidelity. Data include images of Hela cells transfected with Vim-T4 ad Vim-T5 knockdown contructs; images of dual fluorescence assay; data analysis for knockdown experiments, Western blotting analysis of vimentin-knockdown in Hela cells, knockdown of endogenous lamin A/C in Hela cells; quantification of lamin A/C knockdown in Hela cells; dual fluorescence assay of human vimentin genes using three plasmids; Western blotting of Arp3 knockdown in SCC9 cells.

All files can be opened using LibreOffice or OpenOffice. Some of the ODS files have a minor problem when opening them in Excel in the Windows computers. Excel will give warning about some unreadable contents. By choosing the repair option, the file contents will be shown without changes compared to the original data. Detailed explanation is given in the “Explanation file”.

More information

• DOI: 10.5281/zenodo.8296

Subjects

• RNA interference, reporter assay, fluorescent proteins, single-cell

Dates

• Publication date: 2014
• Issued: February 03, 2014

Rights

• https://creativecommons.org/publicdomain/zero/1.0/legalcode Creative Commons Zero v1.0 Universal
• info:eu-repo/semantics/openAccess Open Access

Format

electronic resource

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