Title: Regulation of gastric acid secretion in situ by an endogenous activator protein: Studies with activator-specific antibody
Type Dataset Tushar K. Ray, Jyotirmoy Nandi, Larry F. Lemanski and Swapan K. Ghosh (2013): Regulation of gastric acid secretion in situ by an endogenous activator protein: Studies with activator-specific antibody. Zenodo. Dataset. https://zenodo.org/record/7093
Links
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Summary
Monospecific polyclonal antibody was raised against a homogenous preparation of endogenous activator protein (HAF) for the gastric H+,K+-ATPase system. Antibody was used to assess the regulatory role of the HAF in gastric acid secretion by isolated rabbit glands in situ. Immunohistochemical studies revealed aredistribution of theHAFtowards discrete intracellular zones following stimulation of the glands with histamine. The antibody, when inserted into the stimulated gastric glands by digitonin permeabilization, could effectively block the acid forming ability of the cells. The data offers, for the first time, some concrete in situ evidence for the role of the HAF as an intracellular regulator of gastric H+ transport .
More information
- DOI: 10.5281/zenodo.7093
Subjects
- Gastric H, K-ATPase, Endogenous Activator (HAF), Pure HAF, Monospecific anti-HAF antibody, Gastric acid secretion, Rabbit Gastric Glands, Digitonin permeabilization, 14C-aminopyrene uptake
Dates
- Publication date: 2013
- Issued: September 03, 2013
Notes
Other: The manuscript was written (in the early 1990) soon after the data were collected, but never submitted to a journal with the hope of getting better gel picture and fluorescent micrograph which did not materialize due to unavoidable circumstances.Rights
- https://opendefinition.org/licenses/dsl Design Science License
- info:eu-repo/semantics/openAccess Open Access
Format
electronic resource
Relateditems
| Description | Item type | Relationship | Uri |
|---|---|---|---|
| IsPartOf | https://zenodo.org/communities/zenodo |