Title: Dataset related to the article "Evidence of SARS-CoV-2 transcriptional activity in cardiomyocytes of COVID-19 patients without clinical signs of cardiac involvement"

Type Dataset Perrucci, Gianluca Lorenzo, Bulfamante, Gaetano Pietro, Monica Falleni, Sommariva, Elena, Tosi, Delfina, Martinelli, Carla, Songia, Paola, Poggio, Paolo, Carugo, Stefano, Pompilio, Giulio (2020): Dataset related to the article "Evidence of SARS-CoV-2 transcriptional activity in cardiomyocytes of COVID-19 patients without clinical signs of cardiac involvement". Zenodo. Dataset. https://zenodo.org/record/3974891

Authors: Perrucci, Gianluca Lorenzo (Centro Cardiologico Monzino-IRCCS) ; Bulfamante, Gaetano Pietro (University of Milan) ; Monica Falleni (University of Milan) ; Sommariva, Elena (Centro Cardiologico Monzino-IRCCS) ; Tosi, Delfina (University of Milan) ; Martinelli, Carla (University of Milan) ; Songia, Paola (Centro Cardiologico Monzino-IRCCS) ; Poggio, Paolo (Centro Cardiologico Monzino-IRCCS) ; Carugo, Stefano (University of Milan) ; Pompilio, Giulio (Centro Cardiologico Monzino-IRCCS) ;

Links

• Item record in Zenodo
• Digital object URL

Summary

This record contains raw data related to the article "Evidence of SARS-CoV-2 transcriptional activity in cardiomyocytes of COVID-19 patients without clinical signs of cardiac involvement"

Article abstract:

Background. Cardiovascular complication in patients affected by coronavirus respiratory disease (COVID-19) are increasingly recognized. However, although a cardiac tropism of SARS-CoV-2 for inflammatory cells in autopsy heart samples of COVID-19 patients has been reported, the presence of the virus in cardiomyocytes has not been documented yet.

Methods. We investigated for SARS-CoV-2 presence in heart tissue autopsies of 7 consecutive COVID-19 patients deceased for respiratory failure showing no signs of cardiac involvement and with no history of heart disease. Cardiac autopsy samples were analysed by digital PCR, Western blot, immunohistochemistry, immunofluorescence, RNAScope, and transmission electron microscopy assays.

Results. The presence of SARS-CoV-2 into cardiomyocytes was invariably detected. A variable pattern of cardiomyocytes injury was observed, spanning from absence of cell death and subcellular alterations hallmarks to intracellular oedema and sarcomere rupture. In addition, we found active viral transcription in cardiomyocytes by detecting both sense and antisense SARS-CoV-2 spike RNA.

Conclusions. In this analysis of autopsy cases, the presence of SARS-CoV-2 into cardiomyocytes determining a variable pattern of intracellular involvement has been documented. This finding suggest the need of a cardiologic surveillance even in survived COVID-19 patients not displaying a cardiac phenotype, to monitor potential long-term cardiac sequelae.

More information

• DOI: 10.5281/zenodo.3974891
• Language: en

Dates

• Publication date: 2020
• Issued: June 08, 2020

Rights

• info:eu-repo/semantics/restrictedAccess Restricted Access

Format

electronic resource

Relateditems