Title: Dysregulation in mTOR/HIF-1 signaling identified by proteo-transcriptomics of SARS- CoV-2 infected cells - Proteomic data obtained with Huh-7 cells

Type Dataset Sofia Appelberg, Soham Gupta, Anoop T Ambikan, Flora Mikealoff, Akos Vegvari, Sara Svensson Akusjärvi, Rui Benfeitas, Maike Sperk, Marie Ståhlberg, Shuba Krishnan, Kamalendra Singh, Josef M Penninger, Ali Mirazimi, Ujjwal Neogi (2020): Dysregulation in mTOR/HIF-1 signaling identified by proteo-transcriptomics of SARS- CoV-2 infected cells - Proteomic data obtained with Huh-7 cells. Zenodo. Dataset. https://zenodo.org/record/3754719

Authors: Sofia Appelberg (Public Health Agency of Sweden) ; Soham Gupta (Karolinska Institutet) ; Anoop T Ambikan (Karolinska Institutet) ; Flora Mikealoff (Karolinska Institutet) ; Akos Vegvari (Karolinska Institutet) ; Sara Svensson Akusjärvi (Karolinska Institutet) ; Rui Benfeitas (Stockholm University) ; Maike Sperk (Karolinska Institutet) ; Marie Ståhlberg (Karolinska Institutet) ; Shuba Krishnan (Karolinska Institutet) ; Kamalendra Singh (Karolinska Institutet) ; Josef M Penninger (Institute of Molecular Biotechnology of the Austrian Academy of Sciences) ; Ali Mirazimi (Karolinska Institutet) ; Ujjwal Neogi (Karolinska Institutet) ;

Links

• Item record in Zenodo
• Digital object URL

Summary

Cultured human Huh-7 cells were infected with SARS-CoV-2 and harvested after 24, 48 and 72 h. The extracted proteins were processed in triplicates preparing for mass spectrometric analysis. Data acquisition was completed, including control samples of non-infected cells, following isobaric tandem mass tag (TMT) chemical labeling and on-line fractionation of the 12 combined biological replicates. The resulted vendor specific raw files (Thermo Scientific) of 12 fractions are provided.

The data is further analyzed in order to identify regulated proteins upon SARS-CoV-2 infection to understand the underlying biological processes through pathway analysis. Additional details about the study is going to be completed in the manuscript already submitted for publication.

More information

• DOI: 10.5281/zenodo.3754719
• Language: en

Subjects

• mass spectrometry, proteomics, SARS-CoV-2, Huh-7, TMT-Pro labeling

Dates

• Publication date: 2020
• Issued: April 28, 2020

Rights

• https://creativecommons.org/licenses/by/4.0/legalcode Creative Commons Attribution 4.0 International
• info:eu-repo/semantics/openAccess Open Access

Format

electronic resource

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