Title: Single cell and immune repertoire profiling of COVID-19 patients reveal novel therapeutic candidates

Type Dataset Qinghua Jiang (2020): Single cell and immune repertoire profiling of COVID-19 patients reveal novel therapeutic candidates. Zenodo. Dataset. https://zenodo.org/record/3744141

Authors: Qinghua Jiang (Harbin Institute of Technology) ; Qinghua Jiang (Harbin Institute of Technology) ;

Links

• Item record in Zenodo
• Digital object URL

Summary

COVID-19, a novel pneumonia caused by SARS-CoV-2, has rapidly become the largest threat to public health. Recent studies reported lymphocytopenia but also expansions of antigen-specific T/B cells during early-recovery stage. Here we investigated 16 early-recovery patients using scRNA-seq, HLA-genotyping and deep immune repertoire profling. Our analysis revealed 916 COVID-19-specific TCR groups enriched for a novel cytotoxic CD8+ phenotype with both resident memory (ZNF683+) and tissue exit (SIRP5) markers, and identified 114 statistically-confident virus epitopes. Further, we uncovered 374 BCR groups with somatic hypermutations and/or class switch recombinations. Molecular dynamics simulations revealed 15% of the highly expanded groups may serve as neutralizing antibodies against the virus Spike protein. Finally, we discovered that bacterial infection in COVID-19 patients may elevate monocytic myeloidderived suppressor cells via TLR4/NF!B signaling and cause disease aggravation. Together, we expect our findings and immune-receptor datasets to provide immediate therapeutic options against the pandemic of COVID-19.

More information

• DOI: 10.5281/zenodo.3744141

Subjects

• COVID-19, Single cell sequencing, Immune repertoire profiling

Dates

• Publication date: 2020
• Issued: April 08, 2020

Rights

• https://creativecommons.org/licenses/by/4.0/legalcode Creative Commons Attribution 4.0 International
• info:eu-repo/semantics/openAccess Open Access

Format

electronic resource

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