Title: Single particle tracking data for "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"

Type Dataset Lu, Huasong, Yu, Dan, Hansen, Anders S., Ganguly, Sourav, Liu, Rongdiao, Heckert, Alec, Darzacq, Xavier, Zhou, Qiang (2018): Single particle tracking data for "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD". Zenodo. Dataset. https://zenodo.org/record/1215836

Authors: Lu, Huasong (Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720) ; Yu, Dan (Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720) ; Hansen, Anders S. (Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720) ; Ganguly, Sourav (Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720) ; Liu, Rongdiao (School of Pharmaceutical Sciences, Xiamen University, Xiamen, Fujian 361005, China) ; Heckert, Alec (Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720) ; Darzacq, Xavier (Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720) ; Zhou, Qiang (Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720) ;

Links

• Item record in Zenodo
• Digital object URL

Summary

Experimental single-particle tracking (SPT) data supporting "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"

This dataset contains all the raw SPT data reported in "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD" in the form of SPT trajectories. The SPT trajectories are provided in two different formats for convenience: a CSV format and a Matlab format. Both formats are readable by Spot-On: https://spoton.berkeley.edu/

The SPT data contains "fast tracking" spaSPT data (Figure 2d) and this data was analyzed using the Matlab version of Spot-On which can be found and downloaded at: https://gitlab.com/tjian-darzacq-lab/spot-on-matlab

The SPT data also contains "slow tracking" SPT data (Figure 2e).

Full details about the Matlab and CSV formats are provided in the ReadMe files in the associated zip files.

Please see the associated manuscript for a detailed description of how the data was acquired and analyzed. For questions about the data please contact Anders Sejr Hansen at anders.sejr.hansen {at} berkeley {dot} edu.

More information

• DOI: 10.5281/zenodo.1215836
• Language: en

Subjects

• Single-particle tracking, Single molecule, Imaging, Spot-On, Cyclin T1, P-TEFb, RNA Polymerase II

Dates

• Publication date: 2018
• Issued: April 10, 2018

Notes

Rights

• https://creativecommons.org/licenses/by/4.0/legalcode Creative Commons Attribution 4.0 International
• info:eu-repo/semantics/openAccess Open Access

Format

electronic resource

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