Title: Biological data of mussel larvae treated with fluorescent dyes and grown in two pH treatments.

Type Dataset Kapsenberg, Lydia (2021-01-22): Biological data of mussel larvae treated with fluorescent dyes and grown in two pH treatments.. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu. Dataset. https://darchive.mblwhoilibrary.org/handle/1912/26610

Author: Kapsenberg, Lydia ;

Links

• Item record in Woods Hole Open Access Server
• Digital object URL

Summary

Mussel larvae of Mytilus galloprovincialis were grown in two pH treatments (pH 8.1 and 7.4). Larvae were collected for biological measurements of shell field development and calcification at 35 hours post-fertilization (hpf, trochophore stage). Calcein dye was added to the cultures prior to the start of calcification. Calcofluor is live dye and so was added to sampled larvae at 35 hpf for immediate imaging. Confocal microscopy was used for 3D imaging of larvae. Images were processed in ImageJ. Shell field area was determined as the area stained by calcofluor, on one valve. Calcification area was determined as the area stained by calcein, on one valve. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/751258

More information

• URI: https://hdl.handle.net/1912/26610
• DOI: 10.26008/1912/bco-dmo.751258.1
• Language: en_US

Subjects

• Mytilus galloprovincialis, Mussels, PH, Larvae

Dates

• accessioned: January 22, 2021
• available: January 22, 2021
• created: December 20, 2018
• Publication date: January 22, 2021

Notes

Rights

• Creative Commons Attribution 4.0
• https://creativecommons.org/licenses/by/4.0/

Funding Information

Format

electronic resource

Locations

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