Title: Iron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and T5 bacteriophage, 2018 and 2019.

Type Dataset Breitbart, Mya, Buck, Kristen N., Bonnain, Chelsea, Caprara, Salvatore (2020-01-27): Iron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and T5 bacteriophage, 2018 and 2019.. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu. Dataset. https://darchive.mblwhoilibrary.org/handle/1912/25280

Authors: Breitbart, Mya ; Buck, Kristen N. ; Bonnain, Chelsea ; Caprara, Salvatore ;

Links

• Item record in Woods Hole Open Access Server
• Digital object URL

Summary

This data was collected as part of a study investigating the source of iron to bacteriophage (phage for short, or viruses that infect and kill bacteria) progeny. Evidence from a phage that infects E. coli shows iron incorporated into the tail fiber structure. This study aims at identifying whether the source of the iron is environmental or bacterially derived. E. coli bacterial cultures were grown in minimal media spiked with 10 µM 57FeSO4 then infected with phage T4 or T5. The phages were purified by methods of centrifugation, filtration, density-dependent ultracentrifugation, and dialyzing. The resulting phage fractions were quantified by SYBR epifluorescence microscopy and metal concentrations were measured on an ELEMENT XR ICP-MS. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/757485

More information

• URI: https://hdl.handle.net/1912/25280
• DOI: 10.1575/1912/bco-dmo.757485.1
• Language: en_US

Dates

• accessioned: January 27, 2020
• available: January 27, 2020
• created: February 27, 2019
• Publication date: January 27, 2020
• coverage: Note: 20180622 - 20190207 (UTC)

Notes

Rights

• Creative Commons Attribution 4.0
• https://creativecommons.org/licenses/by/4.0/

Funding Information

Format

electronic resource

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