Title: Heterotrophic (non-pigmented) bacteria abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project)

Type Dataset Landry, Michael R. (2019-12-11): Heterotrophic (non-pigmented) bacteria abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu. Dataset. https://darchive.mblwhoilibrary.org/handle/1912/25010

Author: Landry, Michael R. ;

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• Item record in Woods Hole Open Access Server
• Digital object URL

Summary

Non-pigmented bacteria abundances from preserved (0.5% paraformaldehyde) frozen samples run on a Beckman-Coulter Altra flow cytometer after staining with Hoechst 33342 DNA stain and excitation with 200 mW UV and 1W 488 nm laser light. Bacteria discriminated based on DNA content (450±40 nm), as well as forward and 90° side light scatter parameters. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/516204

More information

• URI: https://hdl.handle.net/1912/25010
• DOI: 10.1575/1912/bco-dmo.516204.1
• Language: en_US

Dates

• accessioned: December 11, 2019
• available: December 11, 2019
• created: May 30, 2014
• Publication date: December 11, 2019
• coverage: Note: 20100624 - 20100724 (UTC)

Notes

Rights

• Creative Commons Attribution 4.0
• https://creativecommons.org/licenses/by/4.0/

Funding Information

Format

electronic resource

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