Title: 16S rRNA gene (from RNA) from samples collected on cruise Chikyu-331 in the Okinawa Trough, Japan from September to October 2010

Type Dataset House, Christopher, Brandt, Leah (2019-11-22): 16S rRNA gene (from RNA) from samples collected on cruise Chikyu-331 in the Okinawa Trough, Japan from September to October 2010. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu. Dataset. https://darchive.mblwhoilibrary.org/handle/1912/24891

Authors: House, Christopher ; Brandt, Leah ;

Links

• Item record in Woods Hole Open Access Server
• Digital object URL

Summary

RNA extractions were attempted from sediments at Sites C0014 and C0017 to selectively investigate the active microbial populations at these sites. This study was conducted to supplement the gene-based and metagenomic studies. However, the results from sequenced reverse transcribe 16S rRNA demonstrate the sensitivity of extraction and amplification to external nucleic acids and attest to the challenges in working with low biomass environmental samples. Inconsistencies between primersets and replicates make conclusions about the results of this RNA study extremely tenuous. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/781114

More information

• URI: https://hdl.handle.net/1912/24891
• DOI: 10.1575/1912/bco-dmo.781114.1
• Language: en_US

Dates

• accessioned: November 22, 2019
• available: November 22, 2019
• created: November 05, 2019
• Publication date: November 22, 2019
• coverage: Note: 20100916 - 20100927 (UTC)

Notes

Rights

• Creative Commons Attribution 4.0
• https://creativecommons.org/licenses/by/4.0/

Funding Information

Format

electronic resource

Locations

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