Title: Trichodesmium AHL amendment metatranscriptomic reads accessions and metadata

Type Dataset Dyhrman, Sonya T., Van Mooy, Benjamin A.S. (2019-04-01): Trichodesmium AHL amendment metatranscriptomic reads accessions and metadata. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu. Dataset. https://darchive.mblwhoilibrary.org/handle/1912/23943

Authors: Dyhrman, Sonya T. ; Van Mooy, Benjamin A.S. ;

Links

• Item record in Woods Hole Open Access Server
• Digital object URL

Summary

Trichodesmium is a marine, diazotrophic cyanobacterium that plays a central role in the biogeochemical cycling of carbon and nitrogen. Colonies ubiquitously co-occur with a diverse microbiome of heterotrophic bacteria. Here we show that manipulation of the microbiome with quorum sensing acyl homoserine lactone (AHL) molecules significantly modulated rates of N2 fixation by Trichodesmium collected from the western North Atlantic, with both positive and negative effects of varied magnitude. Changes in Trichodesmium N2 fixation did not clearly correlate with changes in microbiome composition or geochemical patterns. Metatranscriptome sequencing revealed significant changes in the relative abundance of microbiome transcripts encoding metabolic functions consistent with quorum sensing responses in model bacteria. There was little overlap in specific microbiome transcriptional responses to AHL addition between stations, and this variability in microbiome gene expression may underpin the heterogeneous changes in Trichodesmium N2 fixation. These data suggest the microbiome could play a large and previously overlooked role in modulating Trichodesmium N2 fixation. This metadata form describes the metatranscriptomic sequencing reads that were used in the study. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/746654

More information

• URI: https://hdl.handle.net/1912/23943
• DOI: 10.1575/1912/bco-dmo.746654.1
• Language: en_US

Subjects

• Trichodesmium, Metatranscriptome, Quorum sensing, Nitrogen fixation

Dates

• accessioned: April 01, 2019
• available: April 01, 2019
• created: September 21, 2018
• Publication date: April 01, 2019
• coverage: Note: 20140508 - 20140526 (UTC)

Notes

Rights

• Creative Commons Attribution 4.0
• https://creativecommons.org/licenses/by/4.0/

Funding Information

Format

electronic resource

Locations

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